By Mohamed Al-Rubeai (eds.)
Animal cells are the popular “cell factories” for the creation of complicated molecules and antibodies to be used as prophylactics, therapeutics or diagnostics. Animal cells are required for the proper post-translational processing (including glycosylation) of biopharmaceutical protein items. they're used for the creation of viral vectors for gene remedy. significant goals for this remedy contain melanoma, HIV, arthritis, cardiovascular and CNS illnesses and cystic fibrosis. Animal cells are used as in vitro substrates in pharmacological and toxicological experiences. This ebook is designed to function a complete evaluate of animal phone tradition, overlaying the present prestige of either study and purposes. For the scholar or R&D scientist or new researcher the protocols are principal to the functionality of telephone tradition paintings, but a wide figuring out is vital for translation of laboratory findings into the commercial construction. in the large scope of the publication, each one subject is reviewed authoritatively via specialists within the box to provide state of the art choice of present study. an incredible reference quantity on phone tradition learn and the way it affects on creation of biopharmaceutical proteins around the world, the e-book is vital interpreting for everybody operating in phone tradition and is a instructed quantity for all biotechnology libraries.
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Extra resources for Animal Cell Culture
Proc Natl Acad Sci U S A 89:5128–5132 Templeton NS, Roberts DD, Safer B (1997) Efﬁcient gene targeting in mouse embryonic stem cells. Gene Ther 4:700–709 Thomas KR, Capecchi MR (1987) Site-directed mutagenesis by gene targeting in mouse embryoderived stem cells. Cell 51:503–512 Thomas BJ, Rothstein R (1989) Elevated recombination rates in transcriptionally active DNA. Cell 56:619–630 Thomason L, Court DL, Bubunenko M, Costantino N, Wilson H, Datta S, Oppenheim A (2007) Recombineering: genetic engineering in bacteria using homologous recombination.
Nat Rev Cancer 12:51–58 Sandhu U, Cebula M, Behme S, Riemer P, Wodarczyk C, Metzger D, Reimann J, Schirmbeck R, Hauser H, Wirth D (2011) Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells. Nucleic Acids Res 39:e1 24 H. Hauser Santiago Y, Chan E, Liu PQ, Orlando S, Zhang L, Urnov FD, Holmes MC, Guschin D, Waite A, Miller JC, Rebar EJ, Gregory PD, Klug A, Collingwood TN (2008) Targeted gene knockout in mammalian cells by using engineered zinc-ﬁnger nucleases.
Nonetheless, this highly efﬁcient technology provides usually high transfection rates and is one of the methods of choice for both stable and transient transfection at small scale. This is also reﬂected by a wide range of commercial reagents available for this application. Different lipid-based transfection reagents have been studied in comparative studies (also with reagents from the group of cationic polymers). The performance was shown to depend highly on the cell line used for transfection as well as other process parameters (Schlaeger et al.